Characterization of D 5 Antibody Specificity on Establish ed Human Cell Lines
نویسندگان
چکیده
A monoclonal antibody of the 1gM kappa type directed at a determinant on K-562 cells was produced and characterized. This hybridoma antibody. provisionally termed D5. defines a membrane-associated antigen that seems to be restricted. within the hemopoietic system. to the granulocytic cell lineage and possibly also to very immature erythroid and monocytic cells. Within the granulocytic cell lineage. D5 antibody reacts with all differentiation stages starting from the promyelocyte up to and including the mature granulocyte. Normal and malignant myeloblasts are only weakly positive or negative. Within the monocytic cell lineage. only fairly immature malignant cells as represented by cells from two patients with monoblastic leukemia and by two monoblastoid cell lines were found to be strongly D5 positive. Among the mature glass-adherent
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